The book gathers lecture notes of courses given at the 2014 summer school on integrated biology in Les Houches, France, Session CII. It addresses an emerging field ranging from molecules to cells and to organisms. Through examples it presents a new way of thinking using a combination of interdisciplinary and cutting-edge methods, bridging physics and biology beyond current biophysics. Important novel developments are expected in the coming years that may wellintroduce paradigm shifts in biological science. The school had the ambition to prepare participants to become major actors in these breakthroughs. The power of integrated approaches is illustrated through two cases: interactions between viruses and host cells, and flower development. The role of forces in biology, as well as their mathematical modeling, is illustrated in both processes: how they allow flower organs to emerge or how they control membrane fusion during virus budding. The book also underlines the importance of conformational changes and dynamics of proteins particularly during membrane processes. It explains how membraneproteins can be handled and studied by molecular simulations. Finally, the book also contains concepts in cell biology, in thermodynamics and several novel approaches such as in-cell NMR. Altogether, the chapters show how examining a biological system from different viewpoints based on multidisciplinaryaspects often leads to enriching controversial arguments.
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