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Practical Guide to Protein and Peptide Purification for Microsequencing

By: Paul T. Matsudaira (Author)

Ksh 7,920.00
Format :

Format(s): DRM PDF

ISBN-13: 9780080924618

Publisher: Elsevier Science

Imprint: Academic Press

Publication Date: December 2nd, 2012

Country of Publication: US

Language: English

Pages: 184

Physical Edition EAN: 9780124802827

Category: Biochemistry , Proteins , Cellular biology (cytology) , Biotechnology

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  • Description

  • Author Bio

Why a Second Edition?The Second Edition provides practical answers to the general question, "e;How can I obtain useful sequence information from my protein or peptide?"e; rather than the more specific question asked in the first edition, "e;How can I obtain the N-terminal sequence?"e; Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry. Mass spectrophotometric characterization of proteins and peptides N-terminal sequencing of proteins with blocked N termini Internal amino acid sequence analysis after protease digestion in-gel and on-blot Improved microscale peptide purification methods Computer analysis of protein sequences New protocols tested and refined through everyday use in authors' laboratories Updated reference chapter covering all aspects of protein microsequencing

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